Cytotoxicity and potential anti-inflammatory activity of Libidibia ferrea L. extract on RAW264.7 macrophages
DOI:
https://doi.org/10.53660/CONJ-931-K13Palavras-chave:
Inflammation, Nitric Oxide, Cytotoxicity, Herbal MedicineResumo
Libidibia ferrea L, is extensively used in traditional medicine due to its many pharmacological benefits. This study investigated the non-cytotoxic in in-vitro concentration and the anti-inflammatory effects of the hydroalcoholic extract of the bark of L. ferrea in LPS (lipopolysaccharide from Escherichia coli) stimulated RAW 264.7 cells. Different concentrations of L. ferrea (100 µg/mL; 50 µg/mL; 12,5 µg/mL; 6,25 µg/mL; 3,12 µg/mL; 1,56 µg/mL), medium culture (negative control), LPS (positive control) and dexamethasone (standard drug) was applied to 1 µg/mL LPS-induced RAW 264.7 macrophages in vitro for 24 h. Cytotoxicity was investigated using MTT (3-(4.5-dimethylthiazol-2yl|-2.5diphenyltetrazolium bromide) colorimetric assay. The production of nitric oxide (NO) was determined using the Griess Reaction. The extract showed no significant cytotoxicity at concentrations from 1,56 to 100 μg/mL (cell viability > 80%) in the MTT cell viability assay after 24 h of treatment. The minimum anti-inflammatory concentration of L. ferrea extract based on the release of NO by macrophages was 50 and 100 µg/mL (p<0.05), showing better results than the dexamethasone (standard drug) (p<0.05). This study suggests L. ferrea anti-inflammatory properties.
Palavras-chave: Inflammation; Nitric Oxide; Cytotoxicity; Herbal Medicine.
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